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    大鼠磷酸化Tau蛋白(pMAPT/pTAU)酶聯免疫吸附測定試劑盒
    ELK9247
    規格: 價格:
    48 T ¥2240.00
    96 T ¥3200.00

    Overview

    Product name: Rat pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
    Reactivity: Rat
    Alternative Names: pMAPT /pTAU
    Assay Type: Sandwich
    Sensitivity: 12.37pg/mL
    Standard: 2000 pg/mL
    Range: 31.25-2000 pg/mL
    Sample type: serum, plasma, other biological fluids
    Assay length: 3.5h
    Research Area: intracellular signal transduction and neuron's development and viability.
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pMAPT /pTAU(phosphorylated microtubule-associated protein tau). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to pMAPT /pTAU(phosphorylated microtubule-associated protein tau). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pMAPT /pTAU(phosphorylated microtubule-associated protein tau), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pMAPT /pTAU(phosphorylated microtubule-associated protein tau) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    2000.00 2.131 2.044
    1000.00 1.708 1.621
    500.00 1.273 1.186
    250.00 0.806 0.719
    125.00 0.567 0.480
    62.50 0.357 0.270
    31.25 0.235 0.148
    0.00 0.087 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant pMAPT /pTAU and the recovery rates were calculated by comparing the measured value to the expected amount of pMAPT /pTAU in samples.
    Matrix Recovery range Average
    serum(n=5) 80-97% 88%
    EDTA plasma(n=5) 83-99% 95%
    Heparin plasma(n=5) 84-97% 91%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pMAPT /pTAU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 90-99% 95-102% 82-90% 87-98%
    EDTA plasma(n=5) 93-101% 91-99% 83-97% 87-98%
    Heparin plasma(n=5) 86-95% 81-96% 87-96% 85-97%
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