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    大鼠磷酸化細胞外信號調節激酶1/2(pERK1/2)酶聯免疫吸附檢測試劑盒
    ELK9246
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Rat pERK(Phospho Extracellular Signal Regulated Kinase) ELISA Kit
    Reactivity: Rat
    Alternative Names: MAPK1; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; mitogen-activated protein kinase 1
    Assay Type: Sandwich
    Sensitivity: 11.81 pg/mL
    Standard: 2000 pg/mL
    Range: 31.25-2000 pg/mL
    Sample type: Serum, plasma, tissue homogenates and other biological fluids
    Assay length: 3.5h
    Research Area: Regulating translation
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pERK(Phospho Extracellular Signal Regulated Kinase). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to pERK(Phospho Extracellular Signal Regulated Kinase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pERK(Phospho Extracellular Signal Regulated Kinase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pERK(Phospho Extracellular Signal Regulated Kinase) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    2000.00 2.045 1.958
    1000.00 1.663 1.576
    500.00 1.182 1.095
    250.00 0.871 0.784
    125.00 0.539 0.452
    62.50 0.369 0.282
    31.25 0.215 0.128
    0.00 0.087 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant pERK and the recovery rates were calculated by comparing the measured value to the expected amount of pERK in samples.
    Matrix Recovery range Average
    serum(n=5) 80-95% 87%
    EDTA plasma(n=5) 78-92% 85%
    Heparin plasma(n=5) 92-107% 99%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pERK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 87-96% 82-95% 92-105% 87-98%
    EDTA plasma(n=5) 95-102% 89-99% 83-99% 93-102%
    Heparin plasma(n=5) 86-94% 81-95% 98-105% 85-96%
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