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    人抗凝固蛋白抗體(anti-CarP)酶聯免疫吸附檢測試劑盒
    ELK9238
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Human anti-CarP(anti-carbamylated protein) ELISA Kit
    Reactivity: Human
    Alternative Names: anti- CarP
    Assay Type: Sandwich
    Sensitivity: 0.32 ng/mL
    Standard: 50 ng/mL
    Range: 0.79-50 ng/mL
    Sample type: Serum, plasma and other biological fluids.
    Assay length: 3.5h
    Research Area: Biochemical research
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to anti-carbamylated protein(anti-CarP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-carbamylated protein(anti-CarP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain anti-carbamylated protein(anti-CarP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of anti-carbamylated protein(anti-CarP) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    50.00 2.201 2.119
    25.00 1.612 1.530
    12.50 1.193 1.111
    6.25 0.875 0.793
    3.13 0.557 0.475
    1.57 0.357 0.275
    0.79 0.219 0.137
    0.00 0.082 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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