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    人甲狀腺球蛋白(TG-Ab)ELISA Kit
    ELK9250
    規格: 價格:
    48 T ¥2240.00
    96 T ¥3200.00

    Overview

    Product name: Human TG-ab(Thyroglobulin Ab) ELISA Kit
    Reactivity: Human
    Alternative Names: TG AB; TG-AB
    Assay Type: Competitive Inhibition
    Sensitivity: 0.51 ng/mL
    Standard: 100 ng/mL
    Range: 1.57-100 ng/mL
    Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
    Assay length: 2h
    Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroglobulin Ab(TG-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroglobulin Ab(TG-Ab). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroglobulin Ab(TG-Ab) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    100.00 0.227 0.000
    50.00 0.386 0.000
    25.00 0.621 0.000
    12.50 0.861 0.000
    6.25 1.168 0.000
    3.13 1.557 0.000
    1.57 1.796 0.000
    0.00 2.115 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant TG-ab and the recovery rates were calculated by comparing the measured value to the expected amount of TG-ab in samples.
    Matrix Recovery range Average
    serum(n=5) 78-90% 84%
    EDTA plasma(n=5) 85-99% 92%
    Heparin plasma(n=5) 90-105% 97%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TG-ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 87-101% 89-102% 88-103% 83-94%
    EDTA plasma(n=5) 92-105% 89-97% 87-95% 83-96%
    Heparin plasma(n=5) 92-102% 82-93% 83-97% 92-103%
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