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    人去甲腎上腺素(NE)酶聯免疫吸附檢測試劑盒
    ELK9243
    規格: 價格:
    48 T ¥2660.00
    96 T ¥3800.00

    Overview

    Product name: Human NE(Noradrenaline) ELISA Kit
    Reactivity: Human
    Alternative Names: NA; NAd; Norepinephrine; Norepi
    Assay Type: Competitive Inhibition
    Sensitivity: 26.1 pg/mL
    Standard: 5000 pg/mL
    Range: 78.13-5000 pg/mL
    Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
    Assay length: 2h
    Research Area: Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Noradrenaline(NE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Noradrenaline(NE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noradrenaline(NE) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    5000.00 0.235 0.000
    2500.00 0.332 0.000
    1250.00 0.557 0.000
    625.00 0.833 0.000
    312.50 1.127 0.000
    156.25 1.565 0.000
    78.13 1.877 0.000
    0.00 2.132 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.
    Matrix Recovery range Average
    serum(n=5) 87-99% 93%
    EDTA plasma(n=5) 87-99% 93%
    Heparin plasma(n=5) 80-96% 88%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 79-96% 89-101% 88-97% 97-105%
    EDTA plasma(n=5) 81-93% 89-102% 87-95% 85-96%
    Heparin plasma(n=5) 92-102% 81-92% 86-93% 91-103%
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