俄罗斯XXXXXBBBBB


    EN

    產品中心

    牛促甲狀腺素釋放激素(TRH)酶聯免疫吸附檢測試劑盒
    ELK9239
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Cattle TRH(Thyrotropin Releasing Hormone) ELISA Kit
    Reactivity: Human
    Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
    Assay Type: Competitive Inhibition
    Sensitivity: 2.63 pg/mL
    Standard: 500 pg/mL
    Range: 7.82-500 pg/mL
    Sample type: serum, plasma and other biological fluids
    Assay length: 2h
    Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone(TRH) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    500.00 0.189 0.000
    250.00 0.367 0.000
    125.00 0.557 0.000
    62.50 0.833 0.000
    31.25 1.225 0.000
    15.63 1.537 0.000
    7.82 1.863 0.000
    0.00 2.211 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant TRH and the recovery rates were calculated by comparing the measured value to the expected amount of TRH in samples.
    Matrix Recovery range Average
    serum(n=5) 90-105% 97%
    EDTA plasma(n=5) 88-103% 95%
    Heparin plasma(n=5) 95-107% 101%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 88-101% 82-95% 95-104% 93-105%
    EDTA plasma(n=5) 82-101% 91-103% 85-94% 79-96%
    Heparin plasma(n=5) 98-105% 82-93% 95-102% 93-101%
    俄罗斯XXXXXBBBBB