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    通用四氫生物蝶呤(THB)酶聯免疫吸附檢測試劑盒
    ELK9249
    規格: 價格:
    48 T ¥2240.00
    96 T ¥3200.00

    Overview

    Product name: General THB(Tetrahydrobiopterin) ELISA Kit
    Reactivity: General
    Alternative Names: Tetrahydrobiopterin; BH4; Kuvan; Sapropterin
    Assay Type: Competitive Inhibition
    Sensitivity: 0.51 ng/mL
    Standard: 100 ng/mL
    Range: 1.57-100 ng/mL
    Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length: 2h
    Research Area: Genetic diseases
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin (THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin (THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin (THB) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    100.00 0.207 0.000
    50.00 0.369 0.000
    25.00 0.587 0.000
    12.50 0.816 0.000
    6.25 1.215 0.000
    3.13 1.561 0.000
    1.57 1.787 0.000
    0.00 2.233 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant THB and the recovery rates were calculated by comparing the measured value to the expected amount of THB in samples.
    Matrix Recovery range Average
    serum(n=5) 83-97% 86%
    EDTA plasma(n=5) 92-106% 99%
    Heparin plasma(n=5) 86-99% 92%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of THB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 97-105% 87-94% 93-101% 88-95%
    EDTA plasma(n=5) 96-101% 85-97% 90-99% 89-101%
    Heparin plasma(n=5) 85-96% 89-102% 98-102% 81-94%
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