|Product name:||General THB(Tetrahydrobiopterin) ELISA Kit|
|Alternative Names:||Tetrahydrobiopterin; BH4; Kuvan; Sapropterin|
|Assay Type:||Competitive Inhibition|
|Sample type:||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids|
|Research Area:||Genetic diseases|
|Test principle:||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin (THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin (THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin (THB) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.