|Product name:||Rat CD3(Cluster of Differentiation 3)ELISA Kit|
|Alternative Names:||CD3; CD3e antigen; CD3e antigen; epsilon polypeptide; TiT3 complex; CD3e molecule; epsilon; CD3-TCR complex; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex; epsilon subunit of T3; T-cell surface antigen T3; Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE|
|Sample type:||Tissue homogenates and other biological fluids.|
|Research Area:||Immunology; Signal Transduction|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD3(Cluster of Differentiation 3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD3(Cluster of Differentiation 3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD3(Cluster of Differentiation 3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD3(Cluster of Differentiation 3) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.