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    小鼠半胱氨酸蛋白酶抑制劑3(CST3)酶聯免疫吸附檢測試劑盒
    ELK1046
    規格: 價格:
    48 T ¥1680.00
    96 T ¥2400.00

    Overview

    Product name: Mouse CST3(Cystatin 3) ELISA Kit
    Reactivity: Mouse
    Alternative Names: Cys-C; Cystatin C; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage
    Assay Type: Sandwich
    Sensitivity: 0.126 ng/mL
    Standard: 20 ng/mL
    Range: 0.32-20 ng/mL
    Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
    Assay length: 3.5h
    Research Area: Kidney biomarker;Urology;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 3(CST3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cystatin 3(CST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 3(CST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin 3(CST3) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    20.00 2.082 1.895
    10.00 1.761 1.574
    5.00 1.251 1.064
    2.50 0.862 0.675
    1.25 0.503 0.316
    0.63 0.401 0.214
    0.32 0.224 0.037
    0.00 0.187 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant CST3 and the recovery rates were calculated by comparing the measured value to the expected amount of CST3 in samples.
    Matrix Recovery range Average
    serum(n=5) 78-90% 84%
    EDTA plasma(n=5) 89-107% 98%
    Heparin plasma(n=5) 87-99% 93%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CST3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 85-92% 79-96% 87-103% 90-99%
    EDTA plasma(n=5) 88-97% 96-105% 91-103% 89-97%
    Heparin plasma(n=5) 85-94% 87-96% 79-95% 95-102%
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