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    人干擾素α(IFNa)酶聯免疫吸附檢測試劑盒
    ELK1043
    規格: 價格:
    48 T ¥1680.00
    96 T ¥2400.00

    Overview

    Product name: Human IFNa(Interferon Alpha) ELISA Kit
    Reactivity: Human
    Alternative Names: IFNα1; IFL; LeIF D; IFN; IFN-Alpha; IFNα13; IFN-A; IFNαP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
    Assay Type: Sandwich
    Sensitivity: 3 pg/mL
    Standard: 500 pg/mL
    Range: 7.82-500 pg/mL
    Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length: 3.5h
    Research Area: Cytokine;Infection immunity;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha(IFNa). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Alpha(IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha(IFNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha(IFNa) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    500.00 2.274 2.204
    250.00 1.359 1.289
    125.00 0.701 0.631
    62.50 0.365 0.295
    31.25 0.211 0.141
    15.63 0.179 0.109
    7.82 0.087 0.017
    0.00 0.070 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant IFNa and the recovery rates were calculated by comparing the measured value to the expected amount of IFNa in samples.
    Matrix Recovery range Average
    serum(n=5) 83-92% 85%
    EDTA plasma(n=5) 84-108% 98%
    Heparin plasma(n=5) 87-96% 92%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 86-93% 91-98% 87-98% 99-105%
    EDTA plasma(n=5) 89-99% 83-103% 98-104% 96-101%
    Heparin plasma(n=5) 85-95% 84-98% 95-106% 88-97%
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