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    人C反應蛋白(CRP)酶聯免疫吸附檢測試劑盒
    ELK1040
    規格: 價格:
    48 T ¥1680.00
    96 T ¥2400.00

    Overview

    Product name: Human CRP(C Reactive Protein) ELISA Kit
    Reactivity: Human
    Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1
    Assay Type: Sandwich
    Sensitivity: 26.1 pg/mL
    Standard: 4000 pg/mL
    Range: 62.5-4000 pg/mL
    Sample type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
    Assay length: 3.5h
    Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein(CRP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C Reactive Protein(CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein(CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C Reactive Protein(CRP) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    4000.00 2.402 2.341
    2000.00 1.822 1.761
    1000.00 1.148 1.087
    500.00 0.780 0.719
    250.00 0.479 0.418
    125.00 0.269 0.208
    62.50 0.191 0.130
    0.00 0.061 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant CRP and the recovery rates were calculated by comparing the measured value to the expected amount of CRP in samples.
    Matrix Recovery range Average
    serum(n=5) 95-106% 101%
    EDTA plasma(n=5) 84-99% 91%
    Heparin plasma(n=5) 87-102% 94%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 86-93% 91-105% 87-98% 99-105%
    EDTA plasma(n=5) 89-99% 83-103% 98-105% 96-101%
    Heparin plasma(n=5) 85-105% 84-98% 95-102% 88-102%
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