|Product name:||Human CRP(C Reactive Protein) ELISA Kit|
|Alternative Names:||C-RP; PTX1; Pentraxin-Related; Pentraxin 1|
|Sample type:||serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids|
|Research Area:||Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein(CRP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C Reactive Protein(CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein(CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C Reactive Protein(CRP) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (pg/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.