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    小鼠降鈣素基因相關肽(CGRP)酶聯免疫吸附檢測試劑盒
    ELK1038
    規格: 價格:
    48 T ¥1680.00
    96 T ¥2400.00

    Overview 文獻

    Product name: Mouse CGRP(Calcitonin Gene Related Peptide) ELISA Kit
    Reactivity: Mouse
    Alternative Names: CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
    Assay Type: Sandwich
    Sensitivity: 4.68 pg/mL
    Standard: 1000 pg/mL
    Range: 15.63-1000 pg/mL
    Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length: 3.5h
    Research Area: Infection immunity;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;Dermatology;
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gene Related Peptide(CGRP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gene Related Peptide(CGRP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gene Related Peptide(CGRP) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    1000.00 2.168 2.110
    500.00 1.319 1.261
    250.00 0.773 0.715
    125.00 0.418 0.360
    62.50 0.251 0.193
    31.25 0.185 0.127
    15.63 0.122 0.064
    0.00 0.058 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant CGRP and the recovery rates were calculated by comparing the measured value to the expected amount of CGRP in samples.
    Matrix Recovery range Average
    serum(n=5) 78-90% 84%
    EDTA plasma(n=5) 84-97% 90%
    Heparin plasma(n=5) 87-95% 91%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CGRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 83-96% 81-94% 86-98% 92-101%
    EDTA plasma(n=5) 85-96% 86-95% 90-102% 81-90%
    Heparin plasma(n=5) 87-95% 83-96% 92-102% 82-93%
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