|Product name:||Human CGRP(Calcitonin Gene Related Peptide) ELISA Kit|
|Alternative Names:||CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta|
|Sample type:||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids|
|Research Area:||Infection immunity;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;Dermatology;|
|Test principle:||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gene Related Peptide(CGRP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gene Related Peptide(CGRP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gene Related Peptide(CGRP) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (pg/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.