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      大鼠酪氨酸蛋白激酶Fyn(FYN)酶聯免疫吸附檢測試劑盒
      ELK9281
      規格: 價格:
      48 T ¥1960.00
      96 T ¥2800.00

      Overview

      Product name: Rat FYN (Tyrosine-protein kinase Fyn) ELISA Kit
      Reactivity: Rat
      Alternative Names: fyn;FYN
      Standard: 10 ng/mL
      Sample type: Tissue homogenates, cell lysates and other biological fluids.
      Assay length: 3.5h
      Research Area: Enzyme & Kinase;
      Assay Type: Sandwich
      Sensitivity: 0.059 ng/mL
      Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-protein kinase Fyn (FYN). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tyrosine-protein kinase Fyn (FYN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine-protein kinase Fyn (FYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine-protein kinase Fyn (FYN) in the samples is then determined by comparing the OD of the samples to the standard curve.
      Range: 0.16-10 ng/mL

      標準曲線

      Concentration (ng/mL) OD Corrected OD
      10.00 2.135 2.048
      5.00 1.686 1.599
      2.50 1.162 1.075
      1.25 0.757 0.670
      0.63 0.556 0.469
      0.32 0.318 0.231
      0.16 0.235 0.148
      0.00 0.087 0.000

      精密度

      Intra-assay Precision (Precision within an assay):CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

      Inter-assay Precision (Precision between assays):CV%<10%

      Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

      回收率

      Matrices listed below were spiked with certain level of recombinant FYN and the recovery rates were calculated by comparing the measured value to the expected amount of FYN in samples.
      Matrix Recovery range Average
      serum(n=5) 84-96% 90%
      EDTA plasma(n=5) 88-102% 95%
      Heparin plasma(n=5) 96-107% 102%

      線性

      The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FYN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
      Matrix 1:2 1:4 1:8 1:16
      serum(n=5) 87-105% 83-92% 92-105% 92-101%
      EDTA plasma(n=5) 81-99% 94-106% 83-97% 79-96%
      Heparin plasma(n=5) 85-99% 79-93% 81-96% 85-96%
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