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      人羰基化蛋白(PC)酶聯免疫吸附檢測試劑盒
      ELK9275
      規格: 價格:
      48 T ¥1960.00
      96 T ¥2800.00

      Overview

      Product name: Human PC(Protein Carbonyl) ELISA Kit
      Reactivity: Human
      Alternative Names: Protein Carbonyl
      Assay Type: Sandwich
      Sensitivity: 0.119 nmol/mL
      Standard: 20 nmol/mL
      Range: 0.32-20 nmol/mL
      Sample type: Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate
      Assay length: 3.5h
      Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
      Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PC(Protein Carbonyl). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PC(Protein Carbonyl). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PC(Protein Carbonyl), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PC(Protein Carbonyl) in the samples is then determined by comparing the OD of the samples to the standard curve.

      標準曲線

      Concentration (nmol/mL) OD Corrected OD
      20.00 2.179 2.092
      10.00 1.708 1.621
      5.00 1.273 1.186
      2.50 0.871 0.784
      1.25 0.567 0.480
      0.63 0.326 0.239
      0.32 0.236 0.149
      0.00 0.087 0.000

      精密度

      Intra-assay Precision (Precision within an assay):CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

      Inter-assay Precision (Precision between assays):CV%<10%

      Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

      回收率

      Matrices listed below were spiked with certain level of recombinant PC and the recovery rates were calculated by comparing the measured value to the expected amount of PC in samples.
      Matrix Recovery range Average
      serum(n=5) 78-92% 85%
      EDTA plasma(n=5) 92-107% 99%
      Heparin plasma(n=5) 80-95% 87%

      線性

      The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
      Matrix 1:2 1:4 1:8 1:16
      serum(n=5) 85-92% 79-96% 85-99% 83-98%
      EDTA plasma(n=5) 86-96% 81-97% 85-96% 82-96%
      Heparin plasma(n=5) 85-98% 95-104% 93-102% 88-102%
      国产69精品久久久久久妇女迅雷