俄罗斯XXXXXBBBBB


    EN

    產品中心

    通用黃體激素(LH)酶聯免疫吸附檢測試劑盒
    ELK9280
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: General LH(Luteinizing Hormone) ELISA Kit
    Reactivity: General
    Alternative Names: ICSH; Lutropin; Interstitial Cell Stimulating Hormone
    Assay Type: Competitive Inhibition
    Sensitivity: 0.85 mIU/mL
    Standard: 0.85 mIU/mL
    Range: 1.57-100mIU/mL
    Sample type: Serum, plasma and other biological fluids
    Assay length: 2h
    Research Area: Endocrinology;Reproductive science;Hormone metabolism;
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone(LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone(LH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Luteinizing Hormone(LH) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (mIU/mL) OD Corrected OD
    100.00 0.227 0.000
    50.00 0.386 0.000
    25.00 0.689 0.000
    12.50 0.856 0.000
    6.25 1.163 0.000
    3.13 1.592 0.000
    1.57 1.761 0.000
    0.00 2.325 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant LH and the recovery rates were calculated by comparing the measured value to the expected amount of LH in samples.
    Matrix Recovery range Average
    serum(n=5) 94-107% 100%
    EDTA plasma(n=5) 81-97% 89%
    Heparin plasma(n=5) 90-105% 97%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 85-93% 95-106% 87-96% 82-90%
    EDTA plasma(n=5) 92-101% 95-102% 98-106% 87-98%
    Heparin plasma(n=5) 83-95% 84-99% 83-101% 95-104%
    俄罗斯XXXXXBBBBB