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    大鼠肌酐(CTN)酶聯免疫吸附檢測試劑盒
    ELK9274
    規格: 價格:
    48 T ¥2660.00
    96 T ¥3800.00

    Overview

    Product name: Rat CTN(Creatinine) ELISA Kit
    Reactivity: Rat
    Alternative Names: Creatinine
    Assay Type: Competitive Inhibition
    Sensitivity: 0.89 μg/mL
    Standard: 200 μg/mL
    Range: 3.13-200 μg/mL
    Sample type: serum, plasma and other biological fluids
    Assay length: 2h
    Research Area: Signal transduction;Metabolism
    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CTN(Creatinine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CTN(Creatinine). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CTN(Creatinine) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (μg/mL) OD Corrected OD
    200.00 0.193 0.000
    100.00 0.357 0.000
    50.00 0.651 0.000
    25.00 0.861 0.000
    12.50 1.157 0.000
    6.25 1.578 0.000
    3.13 1.769 0.000
    0.00 2.132 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant CTN and the recovery rates were calculated by comparing the measured value to the expected amount of CTN in samples.
    Matrix Recovery range Average
    serum(n=5) 92-102% 97%
    EDTA plasma(n=5) 90-105% 97%
    Heparin plasma(n=5) 85-97% 91%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 87-101% 95-103% 86-97% 92-99%
    EDTA plasma(n=5) 85-94% 97-106% 92-101% 82-90%
    Heparin plasma(n=5) 83-92% 93-102% 89-101% 84-101%
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