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    大鼠結合蛋白2(TRAF2)酶聯免疫吸附檢測試劑盒
    ELK9270
    規格: 價格:
    48 T ¥2660.00
    96 T ¥3800.00

    Overview

    Product name: Rat TRAF2(TNF Receptor-Associated Factor 2) ELISA Kit
    Reactivity: Rat
    Alternative Names: TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3
    Assay Type: Sandwich
    Sensitivity: 25.1 pg/mL
    Standard: 5000 pg/mL
    Range: 78.13-5000 pg/mL
    Sample type: serum, plasma and other biological fluids
    Assay length: 3.5h
    Research Area: Tumor immunity
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAF2(TNF Receptor-Associated Factor 2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TRAF2(TNF Receptor-Associated Factor 2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRAF2(TNF Receptor-Associated Factor 2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TRAF2(TNF Receptor-Associated Factor 2) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    5000.00 2.266 2.183
    2500.00 1.516 1.433
    1250.00 1.135 1.052
    625.00 0.882 0.799
    312.50 0.518 0.435
    156.25 0.387 0.304
    78.13 0.212 0.129
    0.00 0.083 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant TRAF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TRAF2 in samples.
    Matrix Recovery range Average
    serum(n=5) 78-97% 87%
    EDTA plasma(n=5) 81-95% 88%
    Heparin plasma(n=5) 81-95% 88%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRAF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 86-97% 88-95% 89-99% 83-99%
    EDTA plasma(n=5) 98-105% 81-99% 94-106% 83-97%
    Heparin plasma(n=5) 88-96% 92-104% 87-93% 95-106%
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