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    大鼠含Fas關聯死亡域蛋白(FADD)酶聯免疫吸附檢測試劑盒
    ELK9269
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Rat FADD(Fas Associating Death Domain Containing Protein) ELISA Kit
    Reactivity: Rat

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    10.00 2.096 2.001
    5.00 1.545 1.450
    2.50 1.135 1.040
    1.25 0.839 0.744
    0.63 0.556 0.461
    0.32 0.387 0.292
    0.16 0.189 0.094
    0.00 0.095 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant FADD and the recovery rates were calculated by comparing the measured value to the expected amount of FADD in samples.
    Matrix Recovery range Average
    serum(n=5) 82-98% 87%
    EDTA plasma(n=5) 92-107% 99%
    Heparin plasma(n=5) 80-97% 88%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FADD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 79-96% 85-99% 98-105% 92-101%
    EDTA plasma(n=5) 85-105% 81-98% 78-90% 91-99%
    Heparin plasma(n=5) 97-103% 89-103% 89-97% 95-105%
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