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    豬Ⅲ型前膠原氨基端原肽(PIIINP)酶聯免疫吸附檢測試劑盒
    ELK9263
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Pig PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
    Reactivity: Pig
    Alternative Names: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
    Assay Type: Sandwich
    Sensitivity: 2.33 ng/mL
    Standard: 400 ng/mL
    Range: 6.25-400 ng/mL
    Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length: 3.5h
    Research Area: Metabolic pathway;Hepatology;Bone metabolism;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III N-Terminal Propeptide(PIIINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III N-Terminal Propeptide(PIIINP) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    400.00 2.035 1.948
    200.00 1.506 1.419
    100.00 1.140 1.053
    50.00 0.933 0.846
    25.00 0.518 0.431
    12.50 0.313 0.226
    6.25 0.161 0.074
    0.00 0.087 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant PIIINP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIINP in samples.
    Matrix Recovery range Average
    serum(n=5) 92-107% 99%
    EDTA plasma(n=5) 87-99% 93%
    Heparin plasma(n=5) 81-97% 89%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIINP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 96-105% 95-103% 95-103% 84-98%
    EDTA plasma(n=5) 93-102% 87-98% 87-98% 85-96%
    Heparin plasma(n=5) 80-96% 84-98% 91-97% 81-93%
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