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    豬波形蛋白(VIM)酶聯免疫吸附檢測試劑盒
    ELK9261
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Pig VIM(Vimentin) ELISA Kit
    Reactivity: Pig
    Alternative Names: Vimentin
    Assay Type: Sandwich
    Sensitivity: 0.61 ng/mL
    Standard: 100 ng/mL
    Range: 1.57-100 ng/mL
    Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length: 3.5h
    Research Area: Tumor immunity;Developmental science;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin(VIM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vimentin(VIM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vimentin(VIM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vimentin(VIM) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    100.00 2.096 1.999
    50.00 1.607 1.510
    25.00 1.228 1.131
    12.50 0.883 0.786
    6.25 0.516 0.419
    3.13 0.363 0.266
    1.57 0.256 0.159
    0.00 0.097 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant VIM and the recovery rates were calculated by comparing the measured value to the expected amount of VIM in samples.
    Matrix Recovery range Average
    serum(n=5) 78-92% 85%
    EDTA plasma(n=5) 85-99% 92%
    Heparin plasma(n=5) 84-108% 98%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 79-96% 85-99% 79-93% 81-96%
    EDTA plasma(n=5) 85-96% 83-98% 86-96% 81-97%
    Heparin plasma(n=5) 85-96% 85-92% 83-96% 93-102%
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