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    大鼠Kelch樣ECH關聯蛋白1(KEAP1)酶聯免疫吸附檢測試劑盒
    ELK9260
    規格: 價格:
    48 T ¥2660.00
    96 T ¥3800.00

    Overview

    Product name: Rat KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit
    Reactivity: Rat
    Alternative Names: INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19
    Assay Type: Sandwich
    Sensitivity: 0.055 ng/mL
    Standard: 10 ng/mL
    Range: 0.16-10 ng/mL
    Sample type: tissue homogenates, cell lysates and other biological fluids
    Assay length: 3.5h
    Research Area: Signal transduction;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1(KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1(KEAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    10.00 2.135 2.038
    5.00 1.612 1.515
    2.50 1.211 1.114
    1.25 0.882 0.785
    0.63 0.557 0.460
    0.32 0.323 0.226
    0.16 0.258 0.161
    0.00 0.097 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant KEAP1 and the recovery rates were calculated by comparing the measured value to the expected amount of KEAP1 in samples.
    Matrix Recovery range Average
    serum(n=5) 80-97% 88%
    EDTA plasma(n=5) 90-105% 97%
    Heparin plasma(n=5) 89-103% 96%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KEAP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 96-105% 95-103% 95-103% 84-98%
    EDTA plasma(n=5) 93-102% 80-93% 91-100% 95-103%
    Heparin plasma(n=5) 83-92% 85-92% 87-97% 83-96%
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