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    豬抗平滑肌抗體(ASMA)酶聯免疫吸附檢測試劑盒
    ELK9257
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit
    Reactivity: Pig
    Alternative Names: ASM Ab; Anti Smooth Muscle Antibody
    Assay Type: Sandwich
    Sensitivity: 2.9 ng/mL
    Standard: 500 ng/mL
    Range: 7.82-500 ng/mL
    Sample type: tissue homogenates, cell lysates and other biological fluids
    Assay length: 3.5h
    Research Area: Signal transduction
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Smooth Muscle(SM) protein. Standards or samples are added to the appropriate microtiter plate wells that with Smooth Muscle(SM) protein. Then add a biotin-conjugated Smooth Muscle(SM) protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    500.00 2.096 2.008
    250.00 1.668 1.580
    125.00 1.223 1.135
    62.50 0.933 0.845
    31.25 0.577 0.489
    15.63 0.318 0.230
    7.82 0.236 0.148
    0.00 0.088 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant ASMA and the recovery rates were calculated by comparing the measured value to the expected amount of ASMA in samples.
    Matrix Recovery range Average
    serum(n=5) 96-107% 102%
    EDTA plasma(n=5) 94-107% 100%
    Heparin plasma(n=5) 82-95% 88%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASMA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 82-96% 85-98% 95-104% 93-102%
    EDTA plasma(n=5) 88-102% 85-97% 80-92% 81-93%
    Heparin plasma(n=5) 87-101% 96-105% 89-103% 95-103%
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