|Product name:||Human ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit|
|Sample type:||serum, plasma and other biological fluids|
|Research Area:||Cardiovascular biology|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Cardiolipin IgM protein. Standards or samples are added to the appropriate microtiter plate wells that with Cardiolipin IgM protein. Then add a biotin-conjugated Cardiolipin IgG protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Cardiolipin Antibody IgM(ACA-IgM) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Cardiolipin Antibody IgM(ACA-IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.