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    人抗心磷脂抗體IgM(ACA-IgM) ELISA試劑盒
    ELK9254
    規格: 價格:
    48 T ¥1960.00
    96 T ¥2800.00

    Overview

    Product name: Human ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
    Reactivity: Human
    Alternative Names: LTD4
    Assay Type: Sandwich
    Sensitivity: 0.082 ng/mL
    Standard: 20 ng/mL
    Range: 0.31-20 ng/mL
    Sample type: serum, plasma and other biological fluids
    Assay length: 3.5h
    Research Area: Cardiovascular biology
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Cardiolipin IgM protein. Standards or samples are added to the appropriate microtiter plate wells that with Cardiolipin IgM protein. Then add a biotin-conjugated Cardiolipin IgG protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Cardiolipin Antibody IgM(ACA-IgM) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Cardiolipin Antibody IgM(ACA-IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (ng/mL) OD Corrected OD
    20.00 2.258 2.157
    10.00 1.673 1.572
    5.00 1.182 1.081
    2.50 0.928 0.827
    1.25 0.655 0.554
    0.63 0.369 0.268
    0.32 0.252 0.151
    0.00 0.101 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.
    Matrix Recovery range Average
    serum(n=5) 90-105% 97%
    EDTA plasma(n=5) 87-95% 91%
    Heparin plasma(n=5) 80-97% 88%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 93-103% 81-96% 86-93% 82-95%
    EDTA plasma(n=5) 87-105% 83-92% 92-105% 92-101%
    Heparin plasma(n=5) 81-99% 94-106% 83-97% 88-96%
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